We also identified a range of EutM homologues as robust protein nanostructures with diverse architectures and electrostatic surface properties. We recently developed a self-assembling protein scaffold that covalently immobilized SpyTagged enzymes by engineering the bacterial microcompartment protein EutM from Salmonella enterica with a Sp圜atcher domain. Genetically encoded protein-based biomaterials could provide easy-to-use immobilization platforms for biocatalysts. This work provides a modular, easy-to-use immobilization system that can be tailored for the optimal function of biocatalysts of interest.ĪB - Immobilization of enzymes is required for most biocatalytic processes, but chemistries used in enzyme immobilization are limited and can be challenging. Using an alcohol dehydrogenase as model biocatalyst, we show that the scaffolds improve enzyme activity and stability. In this work, we created a modular immobilization platform with tunable surface properties by developing a toolbox of self-assembling, robust EutM-Sp圜atcher scaffolds. N2 - Immobilization of enzymes is required for most biocatalytic processes, but chemistries used in enzyme immobilization are limited and can be challenging. was supported by funding from a Grand Challenge research award from the University of Minnesota. This work was supported by funds provided by Defense Threat Reduction Agency Grant HDTRA1-15-0004 and Defense Advanced Research Projects Agency Contract HR-0038. TEM imaging of scaffolds was conducted using equipment provided by University Imaging Center, University of Minnesota. T1 - Developing a Protein Scaffolding System for Rapid Enzyme Immobilization and Optimization of Enzyme Functions for Biocatalysis This work provides a modular, easy-to-use immobilization system that can be tailored for the optimal function of biocatalysts of interest.", This work provides a modular, easy-to-use immobilization system that can be tailored for the optimal function of biocatalysts of interest.Ībstract = "Immobilization of enzymes is required for most biocatalytic processes, but chemistries used in enzyme immobilization are limited and can be challenging. Immobilization of enzymes is required for most biocatalytic processes, but chemistries used in enzyme immobilization are limited and can be challenging.
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